Journal: Clinical and Experimental Reproductive Medicine

Article Title: Impact of imatinib administration on the mouse ovarian follicle count and levels of intra-ovarian proteins related to follicular quality

doi: 10.5653/cerm.2022.05218

Figure Lengend Snippet: Box-whisker plots to demonstrate relative protein levels within whole ovaries. The ovarian levels of anti-Müllerian hormone (AMH, A), BCL-xL (B), BAX (C), acid sphingomyelinase (A-SMase, D), caspase-3 (E), and α-smooth muscle actin (α-SMA, F). Control, 0.1 mL of normal saline; CP 50, cyclophosphamide 50 mg/kg; CP 75, cyclophosphamide 75 mg/kg; Ima 7.5, imatinib 7.5 mg/kg; Ima 15, imatinib 15 mg/kg. a) p <0.01 when compared to the saline control group by the Mann-Whitney U -test.

Article Snippet: After incubation in a blocking buffer (5% non-fat milk, 0.05% Tween-20 in 20 mM TBS at a pH of 8.0) for 1 hour at room temperature, the blots were incubated overnight at 4°C with appropriate primary antibodies: AMH (1:400, sc-166752; Santa Cruz Biotechnology, Dallas, TX, USA), BCL-xL (1:100, sc-271121; Santa Cruz Biotechnology), BAX (1:300, sc-7480; Santa Cruz Biotechnology), A-SMase (1:200, ab83354; Abcam, Cambridge, UK), cleaved caspase-3 (1:500, 5a1e; Cell Signaling Technology, Danvers, MA, USA), and α-SMA (1:200, sc-53142; Santa Cruz Biotechnology).

Techniques: Whisker Assay, Control, Saline, MANN-WHITNEY